Temperature-dependent regulatory mechanism of larval development of the wax moth (Galleria mellonella).

The mechanisms underlying larval diapause in the wax moth (Galleria mellonella) is one of the most throughly studied aspects. At the low temperature of 18°C, the last instar larvae did not pupate but transferred to 30°C they initiated development and pupation in a circadian manner. Different types of surgical manipulations including head-ligation, nerve cord-severance, implantation of the brain, prothoracic glands, accompanied with ecdysteroid titre measurements indicated that diapausing arrest of larval development at 18°C might be due to the nervous inhibition of their prothoracic glands.